Key result
The distance between Cys-373 of actin and Cys-36 of beta-tropomyosin in reconstituted muscle thin filaments was estimated to be 65 A based on fluorescence energy transfer.
Population
Reconstituted muscle thin filaments
Design
Preclinical
Authors
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Adds structural constraint to thin filament models; extends actin-tropomyosin geometry data but leaves open functional implications.
Fluorescence energy transfer experiments estimate the minimal distance between tropomyosin and the myosin-binding site on actin to be approximately 34.5 A, placing constraints on spatial arrangements of thin filament proteins.
Lin et al. (1983) studied this question. Fluorescence energy transfer was evaluated on Distance between Cys-373 of actin and Cys-36 of beta-tropomyosin. The distance between Cys-373 of actin and Cys-36 of beta-tropomyosin in reconstituted muscle thin filaments was estimated to be 65 A based on fluorescence energy transfer.