What question did this study set out to answer?

This research aims to examine the use-dependent effects of NE-10064 on cardiac repolarization in cardiac tissues.

August 1, 1995Journal of Cardiovascular Pharmacology

Use-Dependent Effects of the Class III Antiarrhythmic Agent NE-10064 (Azimilide) on Cardiac Repolarization

Q: What is the clinical evidence from this study?

Intervention: NE-10064 (azimilide). Primary outcome: Cardiac repolarization (effective refractory period, action potential duration, and ion currents).

View Full Paper

Ask AI

Bookmark

Key result

NE-10064 (azimilide) selectively blocked IKr (IC50 0.4 microM) over IKs (IC50 3 microM) and inhibited ICa in a use-dependent manner in animal cardiac tissues.

Population

Ferret papillary muscles and guinea pig ventricular myocytes

Design

Preclinical

Authors

BFBernard FerminiTexas Tech University NJNancy K. JurkiewiczUnited States Military Academy BJBrian JowUnited States Military Academy

Discussion

Member takes

Implication

Azimilide's ion-channel profile merits human testing; leaves open translation to clinical arrhythmia management.

Key Points

This research aims to examine the use-dependent effects of NE-10064 on cardiac repolarization in cardiac tissues.
Examined NE-10064 effects on ferret papillary muscles and guinea pig ventricular myocytes
Measured effective refractory period, isometric twitch tension, and action potential duration at varying frequencies (1 Hz and 3 Hz)
Analyzed IC50 values for potassium and calcium current blockades.
NE-10064 increased effective refractory period at 1 Hz (greater than at 3 Hz) and decreased isometric twitch tension at 3 Hz.
Prolonged action potential duration at 1 Hz up to 3 microM but slightly decreased at 10 microM.
Blocked IKr with IC50 0.4 microM, and inhibited ICa in a use-dependent manner, greater at 3 Hz.

Structured PICO

Population

Ferret papillary muscles and guinea pig ventricular myocytes

Intervention

NE-10064 (azimilide) at concentrations ranging from 0.3 to 30 microM

Outcome

Effects on effective refractory period (ERP), isometric twitch tension, action potential duration (APD), and specific ion currents (IKr, IKs, IKl, ICa)surrogate

NE-10064 (azimilide) acts as a relatively selective blocker of IKr over IKs and inhibits ICa in a use-dependent manner, explaining its effects on cardiac repolarization.

Cite This Study

Fermini et al. (1995) studied this question. NE-10064 (azimilide) was evaluated on Cardiac repolarization (effective refractory period, action potential duration, and ion currents). NE-10064 (azimilide) selectively blocked IKr (IC50 0.4 microM) over IKs (IC50 3 microM) and inhibited ICa in a use-dependent manner in animal cardiac tissues.

synapsesocial.com/papers/6a6eff02f44fa9f079dc6e19 https://doi.org/10.1097/00005344-199508000-00012

View Full Paper

Ask AI

Bookmark

Use-Dependent Effects of the Class III Antiarrhythmic Agent NE-10064 (Azimilide) on Cardiac Repolarization

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Use-Dependent Effects of the Class III Antiarrhythmic Agent NE-10064 (Azimilide) on Cardiac Repolarization

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider