Key result
The U4 nucleotide at the 3' end of the influenza neuraminidase gene increased the mRNA/vRNA ratio up to 20-fold and the p.f.u./HA ratio 10- to 40-fold compared to the C4 nucleotide.
Population
Two isogenic A/WSN/33 influenza viruses and MDBK cells
Comparison
U4 nucleotide at position 4 of the 3' end of the… vs C4 nucleotide at position 4 of the 3' end of the…
Design
Preclinical
Authors
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Nucleotide variation at influenza vRNA 3' end modulates replication kinetics; leaves open effects on viral fitness or attenuation.
The position 4 nucleotide at the 3' end of the influenza virus NA vRNA is a genetic determinant for the repertoire of surface antigens and regulates transcription, which could inform the design of genetically engineered influenza viruses for vaccination.
Lee et al. (1998) studied Influenza virus infection. U4 nucleotide at the 3' end of the neuraminidase (NA) gene vs. C4 nucleotide at the 3' end of the NA gene was evaluated on Viral RNA synthesis, mRNA synthesis, and HA/NA ratio. The U4 nucleotide at the 3' end of the influenza neuraminidase gene increased the mRNA/vRNA ratio up to 20-fold and the p.f.u./HA ratio 10- to 40-fold compared to the C4 nucleotide.
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