What question did this study set out to answer?

Determine the chromosomal and regional localization of the human gene encoding erythropoietin.

May 8, 2008Cytogenetics and Cell Genetics

Regional assignment of the erythropoietin gene to human chromosome region 7pter→q22

View Full Paper

Ask AI

Bookmark

Authors

PWPaul C. WatkinsHuman Genome Sciences (United States)RERoger L. EddyBoston University NHN HoffmanUniversity of California, Irvine

Discussion

Member takes

Implication

Somatic cell hybrid analysis reveals the localization of the human erythropoietin gene to chromosome region 7pter-q22, providing precise chromosomal mapping for red blood cell regulation research.

Key Points

Determine the chromosomal and regional localization of the human gene encoding erythropoietin.
Analyzed DNA from 45 human-mouse somatic hybrid cell lines containing reduced complements of human chromosomes using PstI restriction enzyme digestion.
Performed Southern blot hybridization using a cloned human erythropoietin cDNA probe and evaluated a cell hybrid bearing a translocated derivative of chromosome 7.
Demonstrated complete cosegregation of the erythropoietin gene with human chromosome 7 across all 45 hybrid cell lines examined.
Mapped the gene regionally to chromosome region 7pter→q22 using a translocation-bearing hybrid, and identified a HindIII restriction fragment length polymorphism in human genomic DNA.

Cite This Study

Watkins et al. (2008) studied this question.

synapsesocial.com/papers/6a6f23f735aa2c282ce08ad6 https://doi.org/10.1159/000132281

View Full Paper

Ask AI

Bookmark

Implication

Somatic cell hybrid analysis reveals the localization of the human erythropoietin gene to chromosome region 7pter-q22, providing precise chromosomal mapping for red blood cell regulation research.

Regional assignment of the erythropoietin gene to human chromosome region 7pter→q22

Discussion

Member takes

Implication

Key Points

Cite This Study

Regional assignment of the erythropoietin gene to human chromosome region 7pter→q22

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider