What question did this study set out to answer?

To determine the occurrence of integrons and map the structural arrangement and diversity of cassette-encoded antibiotic resistance genes in clinical bacterial isolates.

January 1, 1995Antimicrobial Agents and ChemotherapyOpen Access

PCR mapping of integrons reveals several novel combinations of resistance genes

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Authors

CLCéline M. LévesqueUniversity of Toronto LPLucie PichéCegep de Sainte Foy CLChloé LaroseUniversité Laval

Discussion

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Implication

Laboratory investigation reveals novel antibiotic resistance gene arrangements in clinical bacteria, suggesting PCR mapping tracks multiresistance dissemination.

Key Points

To determine the occurrence of integrons and map the structural arrangement and diversity of cassette-encoded antibiotic resistance genes in clinical bacterial isolates.
Screened clinical bacterial isolates using oligonucleotide probes targeting the conserved segments flanking integrons and specific antibiotic resistance genes.
Mapped the sequence, content, and linear order of resistance gene cassettes inserted between integron conserved segments using polymerase chain reaction (PCR).
Integrons were found to be widespread among recently collected clinical bacterial isolates.
PCR mapping uncovered several previously unrecognized combinations and arrangements of antibiotic resistance genes integrated as cassettes within these bacterial integrons.

Cite This Study

Lévesque et al. (1995) studied this question.

synapsesocial.com/papers/6a6f31ce26a7f98052dbee53 https://doi.org/10.1128/aac.39.1.185

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January 1, 1995Antimicrobial Agents and ChemotherapyOpen Access

PCR mapping of integrons reveals several novel combinations of resistance genes

View Full Paper

Ask AI

Bookmark

Authors

CLCéline M. LévesqueUniversity of Toronto LPLucie PichéCegep de Sainte Foy CLChloé LaroseUniversité Laval

Discussion

Member takes

Implication

Laboratory investigation reveals novel antibiotic resistance gene arrangements in clinical bacteria, suggesting PCR mapping tracks multiresistance dissemination.

Key Points

To determine the occurrence of integrons and map the structural arrangement and diversity of cassette-encoded antibiotic resistance genes in clinical bacterial isolates.
Screened clinical bacterial isolates using oligonucleotide probes targeting the conserved segments flanking integrons and specific antibiotic resistance genes.
Mapped the sequence, content, and linear order of resistance gene cassettes inserted between integron conserved segments using polymerase chain reaction (PCR).
Integrons were found to be widespread among recently collected clinical bacterial isolates.
PCR mapping uncovered several previously unrecognized combinations and arrangements of antibiotic resistance genes integrated as cassettes within these bacterial integrons.

Cite This Study

Lévesque et al. (1995) studied this question.

synapsesocial.com/papers/6a6f31ce26a7f98052dbee53 https://doi.org/10.1128/aac.39.1.185

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