Key result
Serotype-specific mAb-DAS-ELISAs demonstrated 94-100% diagnostic specificity and 90-100% diagnostic sensitivity for FMDV serotypes O, A, Asia-1, SAT-1, SAT-2, and SAT-3.
Why the study?
Classical DAS-ELISAs for foot-and-mouth disease virus use polyclonal antibodies that have inconsistent yields and limited large-scale application compared to monoclonal antibodies.
Population
BEI-inactivated FMD antigens and known negative and positive 10% tissue suspensions of different animal origins
Comparison
Monoclonal antibody-based DAS-ELISAs vs classical polyclonal antibody-based DAS-ELISAs
Design
Diagnostic development and validation study
Authors
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mAb-based DAS-ELISA may enhance FMD antigen detection consistency; leaves open field validation before replacing pAb assays.
The developed mAb-DAS-ELISAs offer highly sensitive and specific rapid serotyping for FMDV, potentially replacing classical pAb-based assays.
Kashem et al. (2025) studied Foot-and-mouth disease (FMD). mAb-DAS-ELISA vs. classical DAS-ELISA was evaluated on Diagnostic specificity (DSp) and sensitivity (DSe) for FMDV serotypes. Serotype-specific mAb-DAS-ELISAs demonstrated 94-100% diagnostic specificity and 90-100% diagnostic sensitivity for FMDV serotypes O, A, Asia-1, SAT-1, SAT-2, and SAT-3.
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