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April 14, 2011Virology JournalOpen Access

Establishment of persistent infection with foot-and-mouth disease virus in BHK-21 cells

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Key result

Treatment with 20 mM ammonium chloride successfully established persistent infection of foot-and-mouth disease virus in BHK-21 cells by reducing viral yield and enhancing cell survival.

Population

BHK-21 cells infected with Foot-and-mouth disease virus (FMDV) serotype O

Comparison

Ammonium chloride treatment vs Wild type viruses

Design

Preclinical

Authors

XHXuan HuangYLYong LiHFHui Fang

Discussion

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Overview

Does not support FMDV treatment changes; leaves open whether the model translates beyond cell culture.

Structured PICO

P
Population
In vitro study using BHK-21 cells to establish persistent infection with foot-and-mouth disease virus.
I
Intervention
Ammonium chloride treatment
C
Comparator
Wild type viruses
O
Outcome
Establishment of persistent infection and viral genome mutations

Ammonium chloride treatment successfully establishes persistent FMDV infection in BHK-21 cells, providing an in vitro model to study viral persistence and evolution.

Limitations

  • In vitro model may not fully replicate in vivo mechanisms of viral persistence
  • The exact influences on the virus life cycle of the observed amino acid mutations remain unclear

Cite This Study

Huang et al. (2011) studied Foot-and-mouth disease virus (FMDV) infection. Ammonium chloride vs. No ammonium chloride was evaluated on Establishment of persistent FMDV infection and viral RNA positive rate. Treatment with 20 mM ammonium chloride successfully established persistent infection of foot-and-mouth disease virus in BHK-21 cells by reducing viral yield and enhancing cell survival.

synapsesocial.com/papers/6a6f57198031ec7bb1dbb410https://doi.org/10.1186/1743-422x-8-169
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A Single Amino Acid Substitution in the Capsid of Foot-and-Mouth Disease Virus Can Increase Acid Lability and Confer Resistance to Acid-Dependent Uncoating Inhibition2010 · 47 citations
  2. 2Foot-and-Mouth Disease2004 · 1,849 citations
  3. 3Deterministic, Compensatory Mutational Events in the Capsid of Foot-and-Mouth Disease Virus in Response to the Introduction of Mutations Found in Viruses from Persistent Infections2006 · 28 citations
  4. 4Role of RNA Structure and RNA Binding Activity of Foot-and-Mouth Disease Virus 3C Protein in VPg Uridylylation and Virus Replication2006 · 79 citations
  5. 5Cytopathogenesis and Inhibition of Host Gene Expression by RNA Viruses2000 · 175 citations