October 1, 1980Journal of General Virology

Immunological Studies of the Group B Coxsackieviruses by the Sandwich Enzyme-linked Immunosorbent Assay (ELISA) and Immunoprecipitation

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Population

Group B Coxsackieviruses (purified preparations and crude infected cell extracts) and hamster antisera

Design

Preclinical

Authors

MKMichael G. KatzeUniversity of Washington RCRichard L. CrowellDrexel University

Discussion

Member takes

Implication

Supports VP1-targeted ELISA development for group B Coxsackieviruses; leaves open clinical validation from animal data.

Structured PICO

Population

Group B Coxsackieviruses (purified preparations and crude infected cell extracts) and hamster antisera

Intervention

Microplate double antibody sandwich ELISA, urea disruption, and immunoprecipitation with SDS-PAGE analysis

Outcome

Detection and quantification of group B Coxsackievirus antigens and antibodies, and identification of common antigenic determinantssurrogate

This basic science study demonstrates that a sandwich ELISA can sensitively detect group B Coxsackieviruses and identifies VPI as the major shared antigenic determinant.

Cite This Study

Katze et al. (1980) studied this question.

synapsesocial.com/papers/6a6f575bac440176ef28084f https://doi.org/10.1099/0022-1317-50-2-357

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