Why the study?
Does ELISA accurately detect type-specific IgG antibodies against Coxsackie B viruses compared to neutralization tests?
Does ELISA accurately detect type-specific IgG antibodies against Coxsackie B viruses compared to neutralization tests?
ELISA lacks type-specificity for detecting Coxsackie B virus IgG antibodies in humans, likely due to masking by group-specific antibodies from repeated enterovirus infections.
ELISA should not guide type-specific Coxsackie B diagnosis in patients; leaves open whether optimized assays can overcome group-specific interference.
In tests for IgG antibodies against Coxsackie B viruses in man, the enzyme-linked immunosorbent assay (ELISA) was essentially group-specific and, unlike the type-specific neutralisation test, usually failed to detect rises in antibody titre in paired, acute and convalescent, sera. However, in rabbits immunised against Coxsackie B viruses, ELISA demonstrated both group- and type-specific antibody responses. The lack of type-specificity of ELISA in man is probably because repeated infection with enteroviruses--echoviruses and Coxsackie A as well as Coxsackie B--results in masking of the type-specific antibody response by group-specific antibody.
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Hannington et al. (1983) studied this question.
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