Rules of engagement between αvβ6 integrin and foot-and-mouth disease virus

Figure 1. Apo and holo αvβ6-FMDV complexes. (a) The density for the αvβ6-PanAsia complex determined by cryo-EM. On the left of the image the front half of the density has been cut away so that a cross-section of the binding can be seen. Depth-cueing is used such that colour indicates radius (<110 Å: cyan; 120–140 Å: yellow; >150 Å red). Clouds of red density show the bound integrin. (b) EM structure of αvβ6-O1M, depicted as for a. (c) The structure of PanAsia determined by crystallography. Colouring reflects the different viral proteins, VP1 (blue), VP2 (green) and VP3 (red). One asymmetric unit is outlined. (d) Enlargement of one asymmetric unit coloured as in c. Two conformations of the VP1 GH loop are rendered using a ribbon representation and the RGD motif is depicted with spheres. The conformational change from that seen in reduced O1 virus (PDB:1FOD) (blue) to the integrin bound conformation (cyan) is depicted with an arrow. Some of the residues involved in heparin sulfate (HS) binding are shown as orange spheres and the binding site is labelled. In all panels, one 2-fold, 3-fold and 5-fold axis of icosahedral symmetry has been labelled by a black ellipse, triangle and pentagon,…

Figure 2. αvβ6-FMDV binding modes. Two predominant binding modes are shown for PanAsia (a,b) and for O1M (c,d). The left panel shows a view down onto the capsid surface (just a pentamer of each virus is shown colour-coded as in Fig. 1c). The integrin is drawn in cartoon representation with the alpha-helices rendered as rods; αv is orange and β6 is green. The right hand panels depict orthogonal side-views of the complexes. The integrin is drawn as in the left panel interacting with a protomer of the virus in cartoon style and colour coded as in Fig. 1c with VP4 in yellow. The FMDV VP1 GH loop, in blue, can be seen interacting with the integrin. The αv N-linked sugar, which forms an additional attachment to the virus, is drawn in magenta (marked with an arrow in a). The similarity in the binding mode ‘A' between the two viruses is evident.

Figure 3. αvβ6-FMDV binding modes. (a–d) The two predominant binding modes are shown for O PanAsia (a,b) and for O1M (c,d) as depicted in Fig. 2 but enlarged to show the electron density for the integrin component.

Figure 4. Close-up of mode A binding of αvβ6 to FMDV. (a) overview in wall-eye stereo of the binding to O PanAsia (b–e) close-ups of the GH loops and N-linked carbohydrate density are shown for O PanAsia (b,c) and for O1M (d,e).

Figure 5. The major binding mode. (a) The schematic shows the change in conformation of the integrin from the ‘closed' state (left) to the ‘open' (right) as visualized in the capsid interaction. (b) Side-view showing an FMDV biological protomer drawn in cartoon format with the proteins coloured by chain as in Fig. 1c. The integrin subunits are drawn in ribbon representation and coloured as in Fig. 2. Key interacting residues are drawn as sticks. The sugar is drawn as sticks in magenta. (c) Close-up of the VP1 GH loop/integrin interactions shown in a with the protein backbone drawn as a ribbon and the interacting side-chains as sticks. The magnesium ion expected to coordinate the MIDAS interaction is included as a grey sphere. (d) Close-up of the sugar interactions shown in a with the proteins depicted as in b.