September 1, 1984Journal of General Virology

Inactivation of Foot-and-Mouth Disease Virus Vaccine Strains by Activation of Virus-associated Endonuclease

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Why the study?

Does incubation with high concentrations of monovalent cations at pH 8.5 safely and effectively inactivate FMDV vaccine strains?

Structured PICO

Does incubation with high concentrations of monovalent cations at pH 8.5 safely and effectively inactivate FMDV vaccine strains?

Population

Foot-and-mouth disease virus (FMDV) vaccine strains (unfractionated viral suspension or purified virions), tested in suckling mice or tissue culture

Intervention

Incubation at 37 degrees C in the presence of high concentrations of monovalent cations (K+, Cs+ or NH4+) at pH 8.5 to activate FMDV endonuclease

Comparator

Untreated controls and other inactivating agents (e.g., binary ethyleneimine)

Outcome

Viral inactivation (residual infectivity, 140S particle amounts, sedimentation coefficient, electrophoretic patterns, viral RNA degradation)surrogate

A new, safer method for inactivating FMDV vaccine strains using monovalent cations at pH 8.5 effectively eliminates infectivity while preserving viral capsid integrity.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

May enable safer FMDV vaccine inactivation in preclinical models; leaves open translation to commercial production.

Cite This Study

Scodeller et al. (1984) studied this question.

synapsesocial.com/papers/6a6f57d8febe604dd7084a8c https://doi.org/10.1099/0022-1317-65-9-1567

View Full Paper

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Inactivation of Foot-and-Mouth Disease Virus Vaccine Strains by Activation of Virus-associated Endonuclease

Structured PICO

Discussion

Implication

Cite This Study

Inactivation of Foot-and-Mouth Disease Virus Vaccine Strains by Activation of Virus-associated Endonuclease

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider