What question did this study set out to answer?

To evaluate an automated, clinical-grade protocol for the expansion and functional enhancement of regulatory T cells to prevent graft-versus-host disease in mismatched transplantation.

September 19, 2020Biology of Blood and Marrow TransplantationOpen Access

Clinical-Grade Expanded Regulatory T Cells Are Enriched with Highly Suppressive Cells Producing IL-10, Granzyme B, and IL-35

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Authors

FUFrancesca Ulbar IVIda Villanova RGRaffaella Giancola

Discussion

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Overview

Preclinical study demonstrates automated expansion produces potent regulatory T cells in mouse models, highlighting a viable strategy to prevent graft-versus-host disease.

Key Points

To evaluate an automated, clinical-grade protocol for the expansion and functional enhancement of regulatory T cells to prevent graft-versus-host disease in mismatched transplantation.
Purified CD4+CD25+ regulatory T cells via CliniMACS and expanded 100 × 10^6 cells for 14 days using the automated CliniMACS Prodigy System.
Characterized phenotype, cytokine expression, and suppressive capacity in vitro by flow cytometry, cell sorting, and mRNA quantification.
Tested in vivo efficacy against graft-versus-host disease (GVHD) in an NSG mouse model co-infused with conventional T cells.
Culture expanded starting cells to 684 ± 279 × 10^6, increasing FoxP3+ purity (82 ± 8%), suppressive activity (75 ± 12%), and marker expression for CD39 (58.1 ± 13.3%, P < .05), CTLA4 (85.4 ± 9.8%, P < .01), and TIM3 (29 ± 16%, P < .05).
Analysis demonstrated significant upregulation of CD39, IL-10, granzyme B, and IL-35 mRNA (P < .05), with isolated TIM3+ populations showing highest IL-10 and granzyme B levels (P < .01).
Mice receiving conventional T cells alone died of GVHD, whereas mice co-infused with expanded regulatory T cells survived without GVHD.

Cite This Study

Ulbar et al. (2020) studied this question.

synapsesocial.com/papers/6a6f57e3a528af2d65c2f1be https://doi.org/10.1016/j.bbmt.2020.08.034

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September 19, 2020Biology of Blood and Marrow TransplantationOpen Access

Clinical-Grade Expanded Regulatory T Cells Are Enriched with Highly Suppressive Cells Producing IL-10, Granzyme B, and IL-35

View Full Paper

Ask AI

Bookmark

Authors

FUFrancesca Ulbar IVIda Villanova RGRaffaella Giancola

Discussion

Member takes

Overview

Preclinical study demonstrates automated expansion produces potent regulatory T cells in mouse models, highlighting a viable strategy to prevent graft-versus-host disease.

Key Points

To evaluate an automated, clinical-grade protocol for the expansion and functional enhancement of regulatory T cells to prevent graft-versus-host disease in mismatched transplantation.
Purified CD4+CD25+ regulatory T cells via CliniMACS and expanded 100 × 10^6 cells for 14 days using the automated CliniMACS Prodigy System.
Characterized phenotype, cytokine expression, and suppressive capacity in vitro by flow cytometry, cell sorting, and mRNA quantification.
Tested in vivo efficacy against graft-versus-host disease (GVHD) in an NSG mouse model co-infused with conventional T cells.
Culture expanded starting cells to 684 ± 279 × 10^6, increasing FoxP3+ purity (82 ± 8%), suppressive activity (75 ± 12%), and marker expression for CD39 (58.1 ± 13.3%, P < .05), CTLA4 (85.4 ± 9.8%, P < .01), and TIM3 (29 ± 16%, P < .05).
Analysis demonstrated significant upregulation of CD39, IL-10, granzyme B, and IL-35 mRNA (P < .05), with isolated TIM3+ populations showing highest IL-10 and granzyme B levels (P < .01).
Mice receiving conventional T cells alone died of GVHD, whereas mice co-infused with expanded regulatory T cells survived without GVHD.

Cite This Study

Ulbar et al. (2020) studied this question.

synapsesocial.com/papers/6a6f57e3a528af2d65c2f1be https://doi.org/10.1016/j.bbmt.2020.08.034

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