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June 1, 1987Proceedings of the National Academy of SciencesOpen Access

Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinases.

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Population

In vitro translation system using plasmids containing the P1 or P1-P2 regions of the poliovirus genome

Comparison

Exogenous proteinase 3C and 2A from… vs Absence of infected-cell extract

Design

Preclinical

Authors

MNMartin J.H. NicklinUniversity of SheffieldHKHans‐Georg KräusslichHeidelberg UniversityHTHidemi ToyodaMie University

Discussion

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Implication

Provides in vitro assay for poliovirus proteinases; leaves open extension to in vivo models or antivirals.

Structured PICO

P
Population
In vitro translation system using plasmids containing the P1 or P1-P2 regions of the poliovirus genome
I
Intervention
Exogenous proteinase 3C and 2A from poliovirus-infected cells
C
Comparator
Absence of infected-cell extract
O
Outcome
Processing of P1 and P1-P2 precursorssurrogate

The study demonstrates an in vitro system for assaying poliovirus proteinases 2A and 3C, confirming 2A as the second polioviral proteinase.

Cite This Study

Nicklin et al. (1987) studied this question.

synapsesocial.com/papers/6a6f58fd75498292b708be3chttps://doi.org/10.1073/pnas.84.12.4002
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Inhibition of HeLa cell protein synthesis following poliovirus infection correlates with the proteolysis of a 220,000-dalton polypeptide associated with eucaryotic initiation factor 3 and a cap binding protein complex.1982 · 592 citations
  2. 2Genetic complementation among poliovirus mutants derived from an infectious cDNA clone1986 · 129 citations
  3. 3Translation of Encephalomyocarditis Virus RNA in vitro Yields an Active Proteolytic Processing Enzyme1978 · 153 citations
  4. 4Identification of the initiation site of poliovirus polyprotein synthesis1982 · 89 citations
  5. 5Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the P3 region1986 · 121 citations