Circ₀124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4

31 patients with acute myocardial infarction (AMI) diagnosed by angiography (excluding severe liver and kidney dysfunction, blood system diseases, cancer, and infectious diseases) and 25 healthy control volunteers. In vitro model: Human cardiomyocytes (AC16) cultured under hypoxia (1% O2).

In vitro: Hypoxia treatment (1% O2 for 24 h), lentivirus short hairpin RNA targeting circ_0124644 (sh-circ_0124644), miR-590-3p mimic/inhibitor, and pcDNA SOX4 overexpression vector.

In vitro: Normoxia (21% O2), control shRNA (sh-NC), control miRNA (miR-NC, anti-NC), and empty pcDNA vector. In vivo: Healthy control volunteers.

Expression levels of circ_0124644, miR-590-3p, and SOX4; cell viability, cell cycle progression, apoptosis, and oxidative stress markers (LDH, MDA, SOD, CAT).surrogate