What question did this study set out to answer?

To determine whether nitric oxide mediates the mitogenic and proliferative effects of vascular endothelial growth factor on coronary postcapillary venular endothelial cells.

January 1, 1996AJP Heart and Circulatory Physiology

Nitric oxide mediates mitogenic effect of VEGF on coronary venular endothelium

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To determine whether nitric oxide mediates the mitogenic and proliferative effects of vascular endothelial growth factor on coronary postcapillary venular endothelial cells.
Assessed cell proliferation and DNA synthesis in cultured microvascular endothelial cells isolated from coronary postcapillary venules following VEGF exposure.
Measured endothelial proliferation and cyclic GMP accumulation in the presence and absence of nitric oxide synthase inhibitors and superoxide dismutase.
VEGF stimulated a dose-dependent increase in endothelial cell proliferation and DNA synthesis, which was suppressed by pretreatment with nitric oxide synthase inhibitors.
VEGF exposure elicited significant increments in cyclic GMP levels, an effect potentiated by superoxide dismutase and abolished by nitric oxide synthase inhibitors.

Structured PICO

Population

Cultured microvascular endothelium isolated from coronary postcapillary venules

Intervention

Vascular endothelial growth factor (VEGF)

Comparator

Presence versus absence of nitric oxide (NO) synthase blockers

Outcome

Cell proliferation, DNA synthesis, and guanosine 3',5'-cyclic monophosphate (cGMP) levelssurrogate

VEGF stimulates proliferation of coronary postcapillary endothelial cells through the production of nitric oxide and subsequent cGMP accumulation.

Discussion

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Implication

Supports VEGF-NO-cGMP axis in coronary endothelial proliferation; hypothesis-generating for angiogenesis therapies, requires human validation.

Cite This Study

Morbidelli et al. (1996) studied this question.

synapsesocial.com/papers/6a6f6a16660549caf2c3f613 https://doi.org/10.1152/ajpheart.1996.270.1.h411

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Implication

Supports VEGF-NO-cGMP axis in coronary endothelial proliferation; hypothesis-generating for angiogenesis therapies, requires human validation.

Nitric oxide mediates mitogenic effect of VEGF on coronary venular endothelium

Key Points

Structured PICO

Discussion

Implication

Cite This Study

Nitric oxide mediates mitogenic effect of VEGF on coronary venular endothelium

Key Points

Structured PICO

Discussion

Implication

Cite This Study

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Also Consider