What question did this study set out to answer?

To determine the structural and physiological consequences of inactivating the Ren-1(d) gene in mouse kidneys.

July 1, 1997Journal of Biological ChemistryOpen Access

Renin-1 Is Essential for Normal Renal Juxtaglomerular Cell Granulation and Macula Densa Morphology

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Population

Mice with inactivated Ren-1(d) gene (Ren-1(d-/-) mice)

Comparison

Inactivation of the Ren-1 gene using a… vs Normal/wild-type mice (implied)

Design

Preclinical

Authors

ACAllan ClarkBrigham Young University MSMatthew G.F. SharpUniversity of Edinburgh SMSteven D. MorleyUniversität Hamburg

Discussion

Member takes

Implication

Ren-1(d) is essential for juxtaglomerular granulation in mice; leaves open its role in human renin-angiotensin regulation.

Key Points

To determine the structural and physiological consequences of inactivating the Ren-1(d) gene in mouse kidneys.
Inactivated the Ren-1(d) gene in mice using a gene-targeting vector with homology arms generated by polymerase chain reaction.
Assessed renal juxtaglomerular cell granulation, macula densa histology, and blood pressure phenotypes in Ren-1(d-/-) mutant mice.
Ren-1(d-/-) mice exhibited altered macula densa morphology and a complete absence of juxtaglomerular cell granulation, despite preserved renin-2 expression.
Mice lacking Ren-1(d) developed sexually dimorphic hypotension, indicating that Ren-1(d) is required for storage granule formation and normal blood pressure maintenance.

Structured PICO

Population

Mice with inactivated Ren-1(d) gene (Ren-1(d-/-) mice)

Intervention

Inactivation of the Ren-1(d) gene using a gene-targeting vector

Comparator

Normal/wild-type mice (implied)

Outcome

Morphology of the macula densa of the kidney distal tubule and juxtaglomerular cell granulationsurrogate

The Ren-1(d) gene is essential for the formation of storage granules in juxtaglomerular cells and normal macula densa morphology in mice.

Cite This Study

Clark et al. (1997) studied this question.

synapsesocial.com/papers/6a6f779a75498292b708dc0a https://doi.org/10.1074/jbc.272.29.18185

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Renin-1 Is Essential for Normal Renal Juxtaglomerular Cell Granulation and Macula Densa Morphology

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Renin-1 Is Essential for Normal Renal Juxtaglomerular Cell Granulation and Macula Densa Morphology

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider