What question did this study set out to answer?

This research aims to introduce a rapid method for determining total protein levels in cerebrospinal fluid.

January 1, 1953Scandinavian Journal of Clinical and Laboratory Investigation1,090 citations

A Micro Biuret Method for Protein Determination Determination of Total Protein in Cerebrospinal Fluid

Key Points

This research aims to introduce a rapid method for determining total protein levels in cerebrospinal fluid.
Developed a colorimetric micromethod for protein determination based on the biuret reaction.
The method analyzes protein concentrations ranging from 10 to 200 mg per cent with a precision of ± 1.5 mg per cent.
An ultra micro modification measures as low as 10 to 400 micrograms of protein.
Successfully achieved a determination of total protein in 1 ml of cerebrospinal fluid.
The micromethod demonstrates a high accuracy for small protein quantities.

Abstract

A rapid and convenient colorimetric method for the determination of small amounts of protein, based on the biuret reaction and readings at 330 mμ, is described. The “micromethod” allows the determination of 10 to 200 mg per cent protein with an acurassy of ± 1.5 mg per cent. An “ultra micro” modification allows determinations of 10 to 400 micrograms protein.The method is applied for the determination of total protein in 1 ml of cerebrospinal fluid.

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