Determination of the Concentration of Protein in Cerebrospinal Fluid With A New Dye‐Binding Method

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The protein concentration of cerebrospinal fluid (CSF) was measured on 50 samples with a new dye-binding method using Coomassie Brilliant Blue G-250 (CBB). The results of this method correlated well (r = 0.99) with those of the trichloroacetic acid-Ponceau S method (TCA-PS). Since the CBC method involves only one step, it is recommended as a simple method of determining protein concentration in CSF.