What question did this study set out to answer?

To examine the local modulation of alpha- and beta-adrenergic vascular responses by nitric oxide and cyclo-oxygenase pathways in the hindlimb circulation of conscious rats.

June 15, 1995The Journal of PhysiologyOpen Access

Local modulation of adrenergic responses in the hindlimb vasculature of the intact conscious rat.

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Population

22 chronically instrumented intact conscious Sprague-Dawley rats

Comparison

Local injection of phenylephrine, clonidine, and… vs Control conditions

Design

Preclinical

Authors

SDStephen E. DiCarloPhiladelphia College of Osteopathic Medicine RPRahul PatilG.S. Science, Arts And Commerce College HCHeidi L. CollinsThe Institute of Addiction Medicine

Discussion

Member takes

Implication

Supports endothelial modulation of adrenergic tone in conscious rats; extends prior observations but leaves open human translation.

Key Points

To examine the local modulation of alpha- and beta-adrenergic vascular responses by nitric oxide and cyclo-oxygenase pathways in the hindlimb circulation of conscious rats.
Sprague-Dawley rats (n = 22) were chronically instrumented with Doppler flow probes on the right common iliac artery and localized arterial catheters.
Assessed dose-response vascular reactions to local intra-arterial injections of phenylephrine, clonidine, and adrenaline before and after treatment with the nitric oxide synthase inhibitor L-NAME or the cyclo-oxygenase inhibitor indomethacin.
Maximal vasoconstrictor responses to phenylephrine were enhanced in the presence of L-NAME (50 ± 6%) and indomethacin (70 ± 9%), with clonidine-induced vasoconstriction likewise enhanced by L-NAME (75.3 ± 4.8%) and indomethacin (50.6 ± 5.7%).
Adrenaline elicited biphasic responses: L-NAME attenuated its vasodilator response by 87 ± 6% and augmented vasoconstriction by 51 ± 7%, whereas indomethacin attenuated vasoconstriction by 71 ± 6%.

Structured PICO

Population

22 chronically instrumented intact conscious Sprague-Dawley rats

Intervention

Local injection of phenylephrine (0.005-0.075 microgram kg-1), clonidine (0.1-0.7 microgram kg-1), and adrenaline (0.02-0.08 microgram kg-1) in the presence of NO synthase inhibitor L-NAME (0.2 mg kg-1) and cyclo-oxygenase inhibitor indomethacin (10 mg kg-1)

Comparator

Control conditions (local injection of the same agonists without inhibitors)

Outcome

Vasoconstrictor and vasodilator responses in the hindlimb vasculaturesurrogate

In conscious rats, local adrenergic vasoconstrictor responses in the hindlimb vasculature are modulated by receptor-mediated release of nitric oxide and prostaglandins.

Cite This Study

DiCarlo et al. (1995) studied this question.

synapsesocial.com/papers/6a6f99f92fdcb5703edb4b8a https://doi.org/10.1113/jphysiol.1995.sp020771

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Local modulation of adrenergic responses in the hindlimb vasculature of the intact conscious rat.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Local modulation of adrenergic responses in the hindlimb vasculature of the intact conscious rat.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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