What question did this study set out to answer?

To describe the physical and kinetic mechanisms governing the enzymatic hydrolysis of insoluble dietary triglycerides by water-soluble lipase at the oil/water interface in the intestine.

May 1, 1966Journal of the American Oil Chemists Society

Enzymatic hydrolysis at an oil/water interface

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Authors

FMF.H. MattsonUniversity of California System RVR.A. VolpenheinProcter & Gamble (India)

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Implication

Biochemical review demonstrates unique kinetics of lipase-mediated triglyceride hydrolysis at the oil-water interface, indicating standard kinetic models fail for intestinal digestion.

Key Points

To describe the physical and kinetic mechanisms governing the enzymatic hydrolysis of insoluble dietary triglycerides by water-soluble lipase at the oil/water interface in the intestine.
Examined the biophysical constraints of two-phase lipid digestion involving water-insoluble triglyceride substrates and water-soluble pancreatic lipase.
Evaluated the impact of interfacial transport mechanisms, fluid agitation, electrolyte levels, and biological surfactants on enzymatic reaction rates.
Standard Michaelis-Menten enzyme kinetics do not apply to intestinal fat digestion because the reaction is confined strictly to the boundary between oil and water phases.
Hydrolysis efficiency is largely determined by physical transport processes, including substrate diffusion toward the interface and removal of digested products away from it.
Surface-active agents—including bile salts, monoglycerides, and fatty acid soaps—along with mechanical mixing and ionic strength, directly govern digestive rate by altering interface properties.

Cite This Study

Mattson et al. (1966) studied this question.

synapsesocial.com/papers/6a6fa20ffebe604dd708820f https://doi.org/10.1007/bf02609675

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Implication

Biochemical review demonstrates unique kinetics of lipase-mediated triglyceride hydrolysis at the oil-water interface, indicating standard kinetic models fail for intestinal digestion.

Enzymatic hydrolysis at an oil/water interface

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Implication

Key Points

Cite This Study

Enzymatic hydrolysis at an oil/water interface

Discussion

Member takes

Implication

Key Points

Cite This Study

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