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June 1, 1993HypertensionOpen Access

A novel angiotensin receptor subtype in rat mesangium. Coupling to adenylyl cyclase.

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Population

rat mesangial cells

Design

Preclinical

Authors

JZJie ZhouMacau University of Science and TechnologyPEPaul ErnsbergerNorthwestern UniversityJDJanice G. DouglasSt. Michael's Hospital

Discussion

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Implication

Characterizes AT receptor subtypes and adenylyl cyclase coupling in rat mesangial cells; hypothesis-generating for human glomerular disease.

Key Points

  • This research aims to identify and characterize angiotensin receptor subtypes in rat mesangial cells and their coupling effects with adenylyl cyclase.
  • Utilized angiotensin receptor antagonists (losartan, PD 123319, CGP 42112A) in radioreceptor and adenylyl cyclase assays.
  • Conducted radioligand binding competition experiments to assess receptor binding sites.
  • Analyzed the impact of angiotensin II on forskolin-stimulated cyclic AMP production.
  • Losartan inhibited 75% of binding at low concentrations (0.1 nM to 0.1 µM) with a subsequent effect on adenylyl cyclase (IC50, 0.5 +/- 0.2 µM).
  • PD 123319 showed inhibition of adenylyl cyclase with an IC50 of 1.2 +/- 0.4 µM but lesser potency compared to radioligand binding predictions.
  • CGP 42112A demonstrated weak activity in both binding and adenylyl cyclase assays (IC50, 5.7 +/- 1.6 µM).

Structured PICO

P
Population
rat mesangial cells
I
Intervention
Angiotensin II receptor antagonists (losartan, PD 123319, CGP 42112A)
O
Outcome
Receptor binding affinity and inhibition of forskolin-stimulated cyclic AMP production (adenylyl cyclase activity)surrogate

The study identifies distinct angiotensin II receptor subtypes in rat mesangial cells and characterizes their differential coupling to adenylyl cyclase.

Cite This Study

Zhou et al. (1993) studied this question.

synapsesocial.com/papers/6a6faeeb26a7f98052dc4cb1https://doi.org/10.1161/01.hyp.21.6.1035
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Angiotensin II receptor subtypes in cultured rat renal mesangial cells1992 · 95 citations
  2. 2Angiotensin II Receptor Heterogeneity1991 · 63 citations
  3. 3Cloning and expression of a novel angiotensin II receptor subtype.1992 · 275 citations
  4. 4Angiotensin II receptor subtypes are coupled with distinct signal-transduction mechanisms in neurons and astrocytes from rat brain.1991 · 245 citations
  5. 5Pertussis toxin blocks angiotensin II‐induced calcium influx but not inositol trisphosphate production in adrenal glomerulosa cell1986 · 73 citations