Polyamines such as spermine and spermidine abolish the inhibitory effect of anti-m7Guo antibodies on the translation of measles virus-induced RNA in vitro.
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Hypothesis-generating for polyamine effects on translation; leaves open any in vivo or clinical relevance.
Antibodies directed against N7-methylguanosine (m7Guo) were prepared and added to a wheat germ cell-free protein-synthesizing system programmed with RNA extracted from monkey cells persistently infected with measles virus. A dose-dependent inhibition of [35S]methionine incorporation was observed when RNA was preincubated with anti-m7Guo immunoglobulins. Antibodies preincubated with m7Guo did not show any inhibiting activity. The inhibitory effect of antibodies was abolished when RNA was preincubated with immunoglobulins in the presence of spermine and spermidine. When polyamines were added to the assay programmed with the IgG-RNA complex, no inhibition was observed.
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Dante et al. (1980) studied this question.
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