March 1, 1986Proceedings of the National Academy of SciencesOpen Access

Poly(riboadenylic acid) preferentially inhibits in vitro translation of cellular mRNAs compared with vaccinia virus mRNAs: possible role in vaccinia virus cytopathology.

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Population

In vitro translation systems programmed with cellular mRNAs and viral mRNAs

Comparison

Poly [poly] at various concentrations vs Untreated controls, or other homopolymers (poly…

Design

Preclinical

Authors

RBRostom BablanianState University of New York ABA K BanerjeeICMR-National Institute of Virology

Discussion

Member takes

Implication

Supports poly(A)-mediated selective inhibition as possible vaccinia shutoff mechanism; hypothesis-generating pending in vivo validation.

Structured PICO

Population

In vitro translation systems (rabbit reticulocyte lysate and wheat germ extract) programmed with cellular mRNAs (HeLa, L-cell) and viral mRNAs (vaccinia virus, encephalomyocarditis virus, reovirus, vesicular stomatitis virus)

Intervention

Poly(riboadenylic acid) [poly(A)] at various concentrations (e.g., <1 microM)

Comparator

Untreated controls, or other homopolymers (poly(C), poly(G), poly(U), poly(dA))

Outcome

Inhibition of mRNA translation (polypeptide synthesis)surrogate

Poly(A) selectively inhibits cellular mRNA translation compared to vaccinia virus mRNA in vitro, suggesting a possible mechanism for vaccinia virus-induced host cell shutoff.

Cite This Study

Bablanian et al. (1986) studied this question.

synapsesocial.com/papers/6a94433e893ee072278c2621 https://doi.org/10.1073/pnas.83.5.1290

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Poly(riboadenylic acid) preferentially inhibits in vitro translation of cellular mRNAs compared with vaccinia virus mRNAs: possible role in vaccinia virus cytopathology.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Poly(riboadenylic acid) preferentially inhibits in vitro translation of cellular mRNAs compared with vaccinia virus mRNAs: possible role in vaccinia virus cytopathology.

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider