What is the clinical evidence from this study?

Study design: Other. Population: Poliovirus infection. Intervention: Poliovirus infection vs. Uninfected host cells. Primary outcome: 5' terminal structure of mRNA.

What question did this study set out to answer?

To determine whether structural modifications to host mRNA explain the shutdown of cellular protein synthesis in poliovirus-infected cells.

May 1, 1976Journal of VirologyOpen Access

Structural difference between the 5' termini of viral and cellular mRNA in poliovirus-infected cells: possible basis for the inhibition of host protein synthesis

View Full Paper

Ask AI

Bookmark

Key result

Poliovirus RNA from polyribosomes was found to have pUp at its 5' end, lacking the methylated cap structures present on intact host mRNA, which may explain the selective translation of viral RNA.

Authors

RFRafael Fernández-MuñozHospital Universitario Ramón y Cajal JDJames DarnellRockefeller University

Discussion

Member takes

Implication

Poliovirus shuts off host translation without mRNA degradation or modification; leaves open precise translational control mechanisms.

Key Points

To determine whether structural modifications to host mRNA explain the shutdown of cellular protein synthesis in poliovirus-infected cells.
Analyzed the structural integrity, average size, poly(A) content, and 5' terminal cap structures of host mRNA isolated from poliovirus-infected HeLa cells.
Assessed poliovirus mRNA isolated from polyribosomes to determine its 5' terminal sequence and modification status.
Host mRNA remained completely intact, retaining its average molecular size, poly(A) content, and 5' terminal methylated cap structures without detectable base modifications at a threshold of 1 in 1,000 bases.
Poliovirus RNA isolated from polyribosomes lacked 5' cap structures and instead possessed a pUp sequence at its 5' end.
Structural divergence at the 5' terminus between capped cellular mRNA and uncapped viral RNA correlates with the selective translation of viral messages during infection.

PICO

Population

Poliovirus infection

Exposure / Comparator

Poliovirus infection vs Uninfected host cells

Primary Outcome

5' terminal structure of mRNA

Cite This Study

Fernández-Muñoz et al. (1976) studied Poliovirus infection. Poliovirus infection vs. Uninfected host cells was evaluated on 5' terminal structure of mRNA. Poliovirus RNA from polyribosomes was found to have pUp at its 5' end, lacking the methylated cap structures present on intact host mRNA, which may explain the selective translation of viral RNA.

synapsesocial.com/papers/6a657ca4a6c99762add5f41b https://doi.org/10.1128/jvi.18.2.719-726.1976

View Full Paper

Ask AI

Bookmark

Structural difference between the 5' termini of viral and cellular mRNA in poliovirus-infected cells: possible basis for the inhibition of host protein synthesis

Discussion

Member takes

Implication

Key Points

PICO

Cite This Study

Structural difference between the 5' termini of viral and cellular mRNA in poliovirus-infected cells: possible basis for the inhibition of host protein synthesis

Discussion

Member takes

Implication

Key Points

PICO

Cite This Study

Also Consider

Also Consider