What question did this study set out to answer?

To determine the metabolic source of amino acids incorporated into poliovirus proteins and measure the time course of viral protein synthesis relative to virion maturation.

January 1, 1960Journal of Biological ChemistryOpen Access

Poliovirus Protein: Source of Amino Acids and Time Course of Synthesis

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Authors

JDJames DarnellRockefeller University LLLeon LevintowNational Institutes of Health

Discussion

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Implication

In vitro cell study reveals poliovirus proteins assemble from host intracellular amino acid pools and outpace virion maturation in HeLa cells, highlighting viral synthesis kinetics.

Key Points

To determine the metabolic source of amino acids incorporated into poliovirus proteins and measure the time course of viral protein synthesis relative to virion maturation.
Propagated Type 1 poliovirus in HeLa S3 suspension cell cultures differentially labeled with carbon-14 L-valine.
Purified the virus using degradative chemical isolation to measure the isotopic content of valine within viral structural proteins.
Poliovirus protein is synthesized de novo from the cell's large intracellular amino acid pool rather than from pre-formed host structural proteins.
Synthesis of viral protein initiates almost simultaneously with the appearance of the first mature virus particles.
Following initiation, viral protein synthesis accelerates rapidly, proceeding at a substantially faster rate than virion maturation.

Cite This Study

Darnell et al. (1960) studied this question.

synapsesocial.com/papers/6a6ff37bfebe604dd708c482 https://doi.org/10.1016/s0021-9258(18)69588-2

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January 1, 1960Journal of Biological ChemistryOpen Access

Poliovirus Protein: Source of Amino Acids and Time Course of Synthesis

View Full Paper

Ask AI

Bookmark

Authors

JDJames DarnellRockefeller University LLLeon LevintowNational Institutes of Health

Discussion

Member takes

Implication

In vitro cell study reveals poliovirus proteins assemble from host intracellular amino acid pools and outpace virion maturation in HeLa cells, highlighting viral synthesis kinetics.

Key Points

To determine the metabolic source of amino acids incorporated into poliovirus proteins and measure the time course of viral protein synthesis relative to virion maturation.
Propagated Type 1 poliovirus in HeLa S3 suspension cell cultures differentially labeled with carbon-14 L-valine.
Purified the virus using degradative chemical isolation to measure the isotopic content of valine within viral structural proteins.
Poliovirus protein is synthesized de novo from the cell's large intracellular amino acid pool rather than from pre-formed host structural proteins.
Synthesis of viral protein initiates almost simultaneously with the appearance of the first mature virus particles.
Following initiation, viral protein synthesis accelerates rapidly, proceeding at a substantially faster rate than virion maturation.

Cite This Study

Darnell et al. (1960) studied this question.

synapsesocial.com/papers/6a6ff37bfebe604dd708c482 https://doi.org/10.1016/s0021-9258(18)69588-2

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