January 1, 1987Nucleic Acids ResearchOpen Access

Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense oligonucleotide analogues having alkyl phosphotriester, methylphosphonate and phosphorothioate linkages

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Population

CV-1 cells transfected with plasmid DNA containing the gene for chloramphenicol acetyl transferase coupled…

Comparison

Antisense oligonucleotide analogues at 30 microM… vs "Normal" phosphodiester oligonucleotides

Design

Preclinical

Authors

CMCarol J. Marcus-SekuraOffice of Technology Transfer AWAmy M. WoernerCenter for Biologics Evaluation and Research KSKazuo ShinozukaGunma University

Discussion

Member takes

Implication

Modified analogues enhance preclinical potency; leaves open translation to human cardiovascular gene therapies.

Structured PICO

Population

CV-1 cells transfected with plasmid DNA containing the gene for chloramphenicol acetyl transferase (CAT) coupled to simian virus 40 regulatory sequences (pSV2CAT) or the human immunodeficiency virus enhancer (pHIVCAT)

Intervention

Antisense oligonucleotide analogues (methylphosphonate, alkyltriester, and phosphorothioate analogues of DNA) at 30 microM concentrations

Comparator

"Normal" phosphodiester oligonucleotides

Outcome

Inhibition of plasmid-directed CAT gene expression/activitysurrogate

Phosphorothioate and methylphosphonate antisense oligonucleotide analogues are more potent inhibitors of targeted gene expression than normal phosphodiester oligonucleotides in vitro.

Cite This Study

Marcus-Sekura et al. (1987) studied this question.

synapsesocial.com/papers/6a6fbdf1660549caf2c449e7 https://doi.org/10.1093/nar/15.14.5749

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Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense oligonucleotide analogues having alkyl phosphotriester, methylphosphonate and phosphorothioate linkages

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Comparative inhibition of chloramphenicol acetyltransferase gene expression by antisense oligonucleotide analogues having alkyl phosphotriester, methylphosphonate and phosphorothioate linkages

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider