Excess antisense RNA from infectious recombinant SV40 fails to inhibit expression of a transfected, interferon‐inducible gene
Key result
A large excess of full-length antisense RNA produced by SV40-based infectious virus constructs failed to inhibit expression of a transfected, interferon-inducible gene in monkey cells.
Population
Monkey CV1 and large T antigen-expressing tsCOS cells stably transfected with a CAT mini-gene controlled by…
Comparison
High copy number of full-length antisense RNA… vs Sense orientation CAT cDNA in SV40 vector, or no…
Design
Preclinical
Authors
Discussion
Loading...
Member takes
Implication
Questions antisense RNA applicability in mammalian systems; leaves open need for refined delivery and targeting strategies.
Structured PICO
P
Population
Monkey CV1 and large T antigen-expressing tsCOS cells stably transfected with a CAT mini-gene controlled by an interferon-inducible promoter
I
Intervention
High copy number of full-length antisense RNA produced by SV40-based infectious virus constructs (with or without interferon treatment)
C
Comparator
Sense orientation CAT cDNA in SV40 vector, or no antisense RNA
O
Outcome
Expression of the transfected CAT mini-genesurrogate
A large excess of full-length antisense RNA is not necessarily sufficient to inhibit gene expression in this viral construct model, even with interferon treatment.
Cite This Study
Kerr et al. (1988) studied this question. SV40-based infectious virus constructs producing antisense RNA vs. Sense orientation virus vector was evaluated on Inhibition of gene expression (CAT mini-gene). A large excess of full-length antisense RNA produced by SV40-based infectious virus constructs failed to inhibit expression of a transfected, interferon-inducible gene in monkey cells.