December 1, 1987Journal of VirologyOpen Access

Site-specific mutations at a picornavirus VP3/VP1 cleavage site disrupt in vitro processing and assembly of capsid precursors

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Population

In vitro model using a cDNA plasmid expressing the viral L-P1-2A capsid precursor of encephalomyocarditis…

Comparison

Site-specific mutations introduced into the… vs Natural cleavage sites (wild-type)

Design

Preclinical

Authors

GPGriffith D. ParksUniversity of Central Florida APAnn C. PalmenbergUniversity of Wisconsin–Madison

Discussion

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Implication

Does not support clinical translation; hypothesis-generating for picornavirus protease specificity in animal models.

Structured PICO

Population

In vitro model using a cDNA plasmid expressing the viral L-P1-2A capsid precursor of encephalomyocarditis virus

Intervention

Site-specific mutations introduced into the Gln-Gly pair at the VP3/VP1 junction (Gln-Ala, Gln-Val, Gln-Glu, Lys-Gly, Lys-Ala, Lys-Val, Lys-Glu, Pro-Gly, and engineered insertion displacing flanking proline)

Comparator

Natural cleavage sites (wild-type)

Outcome

Cleavage activity in assays with protease 3C and formation of 14S pentameric assembly intermediatessurrogate

Specific amino acid sequences at the VP3/VP1 junction are strictly required for protease 3C cleavage and subsequent capsid assembly in encephalomyocarditis virus.

Cite This Study

Parks et al. (1987) studied this question.

synapsesocial.com/papers/6a6fcb4aac440176ef28747e https://doi.org/10.1128/jvi.61.12.3680-3687.1987

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Site-specific mutations at a picornavirus VP3/VP1 cleavage site disrupt in vitro processing and assembly of capsid precursors

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Site-specific mutations at a picornavirus VP3/VP1 cleavage site disrupt in vitro processing and assembly of capsid precursors

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider