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May 1, 1968Journal of Lipid ResearchOpen Access

Modified spray for the detection of phospholipids on thin-layer chromatograms

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Authors

VVV.E. VaskovskyPacific Institute of Bioorganic Chemistry. GB Elyakova Far Eastern Branch of the Russian Academy of SciencesEKE. Ya. KostetskyFar Eastern Federal University

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Implication

Methodological report demonstrates a simplified Dittmer-Lester reagent preparation for detecting phospholipids, suggesting a more efficient visualization protocol for thin-layer chromatography.

Key Points

  • To describe a simplified and practical preparation method for the Dittmer-Lester spray reagent used in thin-layer chromatography.
  • Modified the formulation and preparation steps of the standard Dittmer-Lester detection spray.
  • Applied the modified spray to thin-layer chromatograms to detect and visualize phospholipid fractions.
  • Streamlined the chemical procedure required to produce the Dittmer-Lester reagent.
  • Successfully detected phospholipid bands on thin-layer chromatography plates using the simplified spray preparation.

Cite This Study

Vaskovsky et al. (1968) studied this question.

synapsesocial.com/papers/6a6fcee131a3df8243282086https://doi.org/10.1016/s0022-2275(20)43111-6
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