April 15, 2004Biochemistry

Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Pre-Steady-State Kinetic Analysis of Ribonucleotide Incorporation in the Presence of Mn2+

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Population

RNA-dependent RNA polymerase from poliovirus (3Dpol)

Comparison

Mn2+ as the divalent cation cofactor vs Mg2+ as the divalent cation cofactor

Design

Preclinical

Authors

JAJamie J. ArnoldUniversity of North Carolina at Chapel Hill DGDavid W. GoharaClinical Research Consultants (United States)CCCraig E. CameronUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Mn2+-driven loss of poliovirus polymerase fidelity is hypothesis-generating; leaves open relevance to in vivo replication or antiviral strategies.

Structured PICO

Population

RNA-dependent RNA polymerase from poliovirus (3Dpol)

Intervention

Mn2+ as the divalent cation cofactor

Comparator

Mg2+ as the divalent cation cofactor

Outcome

Kinetic mechanism for single nucleotide incorporation (phosphoryl transfer rate and fidelity)surrogate

Mn2+ diminishes the fidelity of poliovirus RNA polymerase because phosphoryl transfer becomes the rate-limiting step for both correct and incorrect nucleotides, unlike with Mg2+.

Cite This Study

Arnold et al. (2004) studied this question.

synapsesocial.com/papers/6a6fe215f44fa9f079dd60ca https://doi.org/10.1021/bi035213q

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Implication

Mn2+-driven loss of poliovirus polymerase fidelity is hypothesis-generating; leaves open relevance to in vivo replication or antiviral strategies.

Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Pre-Steady-State Kinetic Analysis of Ribonucleotide Incorporation in the Presence of Mn2+

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Poliovirus RNA-Dependent RNA Polymerase (3Dpol): Pre-Steady-State Kinetic Analysis of Ribonucleotide Incorporation in the Presence of Mn2+

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider