February 12, 2008Biochemical JournalOpen Access

Localization of PKA phosphorylation site, Ser2030, in the three-dimensional structure of cardiac ryanodine receptor

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Population

HEK-293 cells expressing GFP-RyR2 fusion proteins (RyR2T2023-GFP and RyR2T2023-GFP)

Design

Preclinical

Authors

PJPeter P. JonesElectrophysiology XMXing MengShandong University of Technology BXBailong XiaoElectrophysiology

Discussion

Member takes

Implication

Structural data exclude direct Ser2030 involvement in FKBP12.6 binding; leaves open indirect PKA effects on RyR2 in cardiac disease models.

Structured PICO

Population

HEK-293 (human embryonic kidney) cells expressing GFP-RyR2 fusion proteins (RyR2T2023-GFP and RyR2(A4860G)T2023-GFP)

Intervention

Insertion of GFP into RyR2-wt and RyR2 mutant A4860G after Thr2023, followed by cryo-electron microscopy and single particle image processing

Outcome

Three-dimensional location of the PKA site Ser2030 in RyR2

The Ser2030 PKA phosphorylation site on the cardiac ryanodine receptor is located in domain 4 and is not directly involved in FKBP12.6 binding, providing structural insights into RyR2 regulation.

Cite This Study

Jones et al. (2008) studied this question.

synapsesocial.com/papers/6a6ff57e31a3df82432840ea https://doi.org/10.1042/bj20071257

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Implication

Structural data exclude direct Ser2030 involvement in FKBP12.6 binding; leaves open indirect PKA effects on RyR2 in cardiac disease models.

Localization of PKA phosphorylation site, Ser2030, in the three-dimensional structure of cardiac ryanodine receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Localization of PKA phosphorylation site, Ser2030, in the three-dimensional structure of cardiac ryanodine receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider