Three-dimensional Localization of Divergent Region 3 of the Ryanodine Receptor to the Clamp-shaped Structures Adjacent to the FKBP Binding Sites

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Population

HEK293 cells expressing GFP-RyR2 fusion protein (RyR2(T1874-GFP)) and wild type RyR2

Comparison

Insertion of green fluorescent protein into the… vs Wild type RyR2

Design

Preclinical

Authors

Discussion

No immediate clinical implications; leaves open DR3 as a target for RyR2 modulation in preclinical models.

Population

HEK293 cells expressing GFP-RyR2 fusion protein (RyR2(T1874-GFP)) and wild type RyR2

Intervention

Insertion of green fluorescent protein (GFP) into the middle of the DR3 region after Thr-1874

Comparator

Wild type RyR2

Outcome

Three-dimensional localization of DR3 in RyR2 structure using cryoelectron microscopysurrogate

The localization of the DR3 region in RyR2 provides a structural basis for its role in excitation-contraction coupling and channel regulation.

Zhang et al. (2003) studied this question.

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