Key result
Insertion of GFP into the divergent region 1 of the ryanodine receptor allowed for its localization to domain 3 (the handle domain) on the three-dimensional structure using cryoelectron microscopy.
Population
HEK293 cells expressing wild type mouse RyR2 (RyR2) and modified RyR2 with GFP inserted into divergent…
Comparison
Internal fusion of green fluorescent protein… vs Wild type mouse RyR2 and native RyR2 purified…
Design
Preclinical
Authors
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Maps RyR divergent region 1 to the handle domain in animal models; leaves open functional relevance for human cardiac calcium handling or disease.
This study successfully mapped the divergent region 1 of the cardiac ryanodine receptor to the 'handle' domain using a novel internal GFP fusion technique and cryoelectron microscopy.
Liu et al. (2002) studied this question. GFP insertion into divergent region 1 of RyR2 vs. Wild type mouse RyR2 was evaluated on Three-dimensional reconstruction and localization of DR1. Insertion of GFP into the divergent region 1 of the ryanodine receptor allowed for its localization to domain 3 (the handle domain) on the three-dimensional structure using cryoelectron microscopy.
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