April 29, 2010Journal of Cell Science

Dynamic, inter-subunit interactions between the N-terminal and central mutation regions of cardiac ryanodine receptor

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Population

HEK293 cells transfected with dual YFP- and CFP-labeled cardiac ryanodine receptor (RyR2) cDNAs

Comparison

Application of RyR2 modulators, domain peptides… vs Cells co-transfected with single CFP and single…

Design

Preclinical

Authors

ZLZheng LiuJilin University RWRuiwu WangElectrophysiology XTXixi TianAffiliated Hospital of Hebei University

Discussion

Member takes

Implication

Extends FRET tools to RyR2 inter-subunit dynamics; leaves open translation to disease models or stabilizers.

Structured PICO

Population

HEK293 cells transfected with dual YFP- and CFP-labeled cardiac ryanodine receptor (RyR2) cDNAs

Intervention

Application of RyR2 modulators, domain peptides mimicking disease-causing mutations, and various drugs

Comparator

Cells co-transfected with single CFP and single YFP labeled RyR2

Outcome

FRET signals reflecting conformational dynamics and inter-subunit interactions between the N-terminal and central mutation regions of RyR2surrogate

FRET analysis of dual-labeled RyR2 reveals inter-subunit interactions between mutation hotspots, offering a method to study channel gating and screen for stabilizing drugs.

Cite This Study

Liu et al. (2010) studied this question.

synapsesocial.com/papers/6a796ac4fbd9051d3b0dc07f https://doi.org/10.1242/jcs.064071

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Dynamic, inter-subunit interactions between the N-terminal and central mutation regions of cardiac ryanodine receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Dynamic, inter-subunit interactions between the N-terminal and central mutation regions of cardiac ryanodine receptor

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider