July 28, 2004Journal of Biological ChemistryOpen Access

Mapping Sites of Potential Proximity between the Dihydropyridine Receptor and RyR1 in Muscle Using a Cyan Fluorescent Protein-Yellow Fluorescent Protein Tandem as a Fluorescence Resonance Energy Transfer Probe

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Structured PICO

Population

Dysgenic (alpha1S-null) and dyspedic (RyR1-null) myotubes

Intervention

Attachment of a cyan and yellow fluorescent protein (CFP-YFP) tandem to sites of the alpha1S and beta1a subunits of the dihydropyridine receptor (DHPR)

Comparator

Free tandem in solution and comparison between dysgenic and dyspedic myotubes

Outcome

Fluorescence resonance energy transfer (FRET) efficiencysurrogate

The C terminus of alpha1S and the N terminus of beta1a of the DHPR may be in close proximity to the ryanodine receptor in skeletal muscle.

Discussion

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Implication

Suggests DHPR-RyR domain proximity in skeletal muscle; leaves open validation in physiological or cardiac models.

Cite This Study

Papadopoulos et al. (2004) studied this question.

synapsesocial.com/papers/6a84a6431505246ba71dd34e https://doi.org/10.1074/jbc.m405317200

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Mapping Sites of Potential Proximity between the Dihydropyridine Receptor and RyR1 in Muscle Using a Cyan Fluorescent Protein-Yellow Fluorescent Protein Tandem as a Fluorescence Resonance Energy Transfer Probe

Structured PICO

Discussion

Implication

Cite This Study

Mapping Sites of Potential Proximity between the Dihydropyridine Receptor and RyR1 in Muscle Using a Cyan Fluorescent Protein-Yellow Fluorescent Protein Tandem as a Fluorescence Resonance Energy Transfer Probe

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider