What question did this study set out to answer?

This research aims to compare the transient kinetics of calcium transport between cardiac and skeletal muscle sarcoplasmic reticulum.

December 1, 1978Journal of Biological ChemistryOpen Access

Transient kinetics of Ca2+ transport of sarcoplasmic reticulum. A comparison of cardiac and skeletal muscle.

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Population

Isolated preparations of cardiac sarcoplasmic reticulum (CSR) and skeletal sarcoplasmic reticulum (SSR)

Comparison

Analysis of phosphorylation, dephosphorylation… vs Comparison between cardiac and skeletal…

Design

Preclinical

Authors

MSMichihiro SumidaUniversity of Miyazaki TWT. WangUniversity of Cincinnati Medical Center FMFrederic MandelUniversity of Maryland, Baltimore

Discussion

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Implication

May underlie slower cardiac relaxation kinetics; leaves open whether these differences extend to human myocardium or guide therapy.

Key Points

This research aims to compare the transient kinetics of calcium transport between cardiac and skeletal muscle sarcoplasmic reticulum.
Studied phosphorlyation, dephosphorylation, and calcium transport using a quench-flow apparatus.
Evaluated rate constants for phosphorylation and dissociation of calcium in cardiac and skeletal muscle preparations.
Both cardiac and skeletal sarcoplasmic reticulum show similar calcium binding properties in the absence of ATP.
The rate of phosphorylation for calcium-preloaded SR is equivalent (t-1/2 - 10 ms) for both types, while CSR has lower dissociation rates (10 s-1) than SSR (15 s-1).
Computer simulations reveal CSR's phosphorylation rate is 12 s-1 compared to SSR's 63 s-1 in high Mg2+ presence.

Structured PICO

Population

Isolated preparations of cardiac sarcoplasmic reticulum (CSR) and skeletal sarcoplasmic reticulum (SSR)

Intervention

Analysis of phosphorylation, dephosphorylation, and calcium transport using a quench-flow apparatus and computer simulation

Comparator

Comparison between cardiac and skeletal sarcoplasmic reticulum

Outcome

Rates of phosphorylation, dephosphorylation, and calcium transportsurrogate

The slower relaxation rate of cardiac muscle compared to fast skeletal muscle may be partly explained by lower rates of calcium dissociation, phosphorylation, and dephosphorylation in the cardiac sarcoplasmic reticulum.

Cite This Study

Sumida et al. (1978) studied this question.

synapsesocial.com/papers/6a6ff94cfebe604dd708c9e4 https://doi.org/10.1016/s0021-9258(17)34244-8

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Transient kinetics of Ca2+ transport of sarcoplasmic reticulum. A comparison of cardiac and skeletal muscle.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Transient kinetics of Ca2+ transport of sarcoplasmic reticulum. A comparison of cardiac and skeletal muscle.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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