What question did this study set out to answer?

This research aims to understand how changing pH impacts the kinetics of Ca2+-Mg2+-ATPase in cardiac and skeletal sarcoplasmic reticulum.

February 1, 1982Circulation ResearchOpen Access

The effect of pH on the transient-state kinetics of Ca2+-Mg2+-ATPase of cardiac sarcoplasmic reticulum. A comparison with skeletal sarcoplasmic reticulum.

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Population

Cardiac and skeletal sarcoplasmic reticulum

Comparison

Acidic pH (pH 6.0) vs Neutral/physiological pH (pH 6.8)

Design

Preclinical

Authors

FMFrederic MandelUniversity of Maryland, Baltimore EKE G KraniasUniversity of Cincinnati Medical Center AGA Grassi de GendeUniversidad Nacional de La Plata

Discussion

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Implication

Acidosis may impair cardiac SR Ca2+-ATPase kinetics more than skeletal; leaves open relevance to ischemic contractile dysfunction.

Key Points

This research aims to understand how changing pH impacts the kinetics of Ca2+-Mg2+-ATPase in cardiac and skeletal sarcoplasmic reticulum.
Used a rapid mixing quench-flow apparatus to measure ATPase kinetics at varying pH levels.
Studied the formation and decomposition rates of the phosphorylated intermediate at pH levels from 6.0 to 7.6.
Compared the effects of pH on cardiac and skeletal sarcoplasmic reticulum preloaded with calcium.
At pH 6.8, cardiac and skeletal SR showed similar formation rates of the phosphorylated intermediate (t1/2 = 10 msec).
At pH 6.0, cardiac SR decomposition half-life increased to 136 msec, while skeletal SR increased to 178 msec (both from lower pH rates).
Acidic conditions had a more severe impact on cardiac SR kinetics compared to skeletal SR.

Structured PICO

Population

Cardiac and skeletal sarcoplasmic reticulum

Intervention

Acidic pH (pH 6.0)

Comparator

Neutral/physiological pH (pH 6.8)

Outcome

Rates of formation and decomposition of the phosphorylated intermediate of the Ca2+-Mg2+-ATPasesurrogate

Acidosis has a more pronounced inhibitory effect on the formation of the phosphorylated intermediate of Ca2+-Mg2+-ATPase in cardiac sarcoplasmic reticulum compared to skeletal muscle.

Cite This Study

Mandel et al. (1982) studied this question.

synapsesocial.com/papers/6a7556ef8d2049c8dd036e10 https://doi.org/10.1161/01.res.50.2.310

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The effect of pH on the transient-state kinetics of Ca2+-Mg2+-ATPase of cardiac sarcoplasmic reticulum. A comparison with skeletal sarcoplasmic reticulum.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The effect of pH on the transient-state kinetics of Ca2+-Mg2+-ATPase of cardiac sarcoplasmic reticulum. A comparison with skeletal sarcoplasmic reticulum.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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