November 1, 1990Biochimica et Biophysica Acta (BBA) - BiomembranesOpen Access

The pH dependence of the cardiac sarcolemmal Ca2+-transporting ATPase: evidence that the Ca2+ translocator bears a doubly negative charge

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Population

Bovine cardiac sarcolemma membrane vesicle preparation

Design

Preclinical

Authors

DDDeborah A. DixonMayo Clinic DHDuncan H. HaynesNational Institutes of Health

Discussion

Member takes

Implication

May alter Ca2+ extrusion in acidotic myocardium; leaves open relevance to human ischemia.

Structured PICO

Population

Bovine cardiac sarcolemma membrane vesicle preparation

Intervention

Varying pH levels (6.0 to 8.5)

Outcome

Maximal velocity (Vmax) and apparent affinity for Ca2+ (1/Km) of the Ca2+-transporting ATPasesurrogate

The study provides mechanistic evidence that the cardiac sarcolemmal Ca2+ pump's affinity for Ca2+ is highly pH-dependent, suggesting a model where the Ca2+ translocator bears a doubly negative charge, which has implications for pump behavior during ischemia and acidosis.

Cite This Study

Dixon et al. (1990) studied this question.

synapsesocial.com/papers/6a7d27758253d2b4939ffe4a https://doi.org/10.1016/0005-2736(90)90164-j

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The pH dependence of the cardiac sarcolemmal Ca2+-transporting ATPase: evidence that the Ca2+ translocator bears a doubly negative charge

Discussion

Member takes

Implication

Structured PICO

Cite This Study

The pH dependence of the cardiac sarcolemmal Ca2+-transporting ATPase: evidence that the Ca2+ translocator bears a doubly negative charge

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider