Profiling of Insulin-Like Growth Factor Binding Proteins (IGFBPs) in Obesity and Their Association With Ox-LDL and Hs-CRP in Adolescents

Figures

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  Figure 1. Differences in IGFBP-1 levels based on (A) sex, (B) age and (C) weight status: (A) IGFBP-1 are significantly lower in females compared to males; N=191 males, 228 females; t=6.18, p<0.001; (B) IGFBP-1 levels are significantly reduced with age; N=178 (10-<12), 143 (12-<13) and 98 (13+); (C) IGFBP-1 is reduced in overweight and obesity compared to normal weight; N= 198 normal weight, 88 overweight, 135 obese. Data is presented as means ± SD. Males and females were compared by t-test for independent samples, whereas, age groups and weight status groups were compared with one-way ANOVA. *p < 0.01; **p < 0.001.

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  Figure 2. Association of Ox-LDL (A–C) and Hs-CRP (D–F) with IGFBPs. (A) Ox-LDL was not associated with IGFBP-1 (β = 0.06; p = 0.40), (B, C) Ox-LDL was negatively associated with IGFBP-3 (β = -0.18; p = 0.001) and IGFBP-7 (β = -0.02; p < 0.001). (D) Hs-CRP was negatively associated with IGFBP-1 (β = -0.30; p = 0.001) (E, F) No significant association of Hs-CRP was found with either IGFBP-3 (β = -0.06; p > 0.05) or IGFBP-7 (β = 0.002; p > 0.05). Data were analyzed by linear regression.

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  Figure 3. Distribution of Ox-LDL (A–C) and Hs-CRP (D–F) in different tertiles of IGFBPs. (A) Levels of Ox-LDL across the three tertiles of IGFBP-1 were not significantly different. (B, C) The levels of Ox-LDL were significantly higher in the lower tertiles of IGFBP-3 and IGFBP-7 compared to the middle and higher tertiles, no differences were observed between Ox-LDL levels in the middle and upper tertiles. (D) Levels of Hs-CRP were significantly higher in the lower tertile of IGFBP-1 compared to the middle and upper tertiles but were not different between the middle and upper tertiles. (E, F) Levels of Hs-CRP across the three tertiles of IGFBP-3 and IGFBP-7 were not significantly different. N=144 in each group. Data is presented as geometric means ± SD. *p < 0.01 by one way ANOVA with Bonferroni post-hoc comparison.