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August 15, 1999Blood

Thrombopoietin-Induced Activation of the Mitogen-Activated Protein Kinase (MAPK) Pathway in Normal Megakaryocytes: Role in Endomitosis

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Population

Cytokine-dependent cell line and primary murine megakaryocytes

Comparison

Thrombopoietin stimulation and MAPK kinase… vs Unstimulated cells or cells without MEK inhibition

Design

Preclinical

Authors

PRPonlapat RojnuckarinChulalongkorn UniversityJDJonathan G. DrachmanSeattle UniversityKKKenneth KaushanskyStony Brook School

Discussion

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Implication

Implicates MAPK in megakaryopoiesis; leaves open whether pathway inhibition affects platelet production in vivo.

Structured PICO

P
Population
Cytokine-dependent cell line (BAF3/Mpl) and primary murine megakaryocytes (including CD41+ selected cells from whole bone marrow)
I
Intervention
Thrombopoietin (TPO) stimulation and MAPK kinase (MEK) inhibitor PD 98059
C
Comparator
Unstimulated cells or cells without MEK inhibition
O
Outcome
MAPK (ERK 1 and 2) phosphorylation, TPO-dependent proliferation, and megakaryocyte ploidysurrogate

The MAPK pathway plays an important role in TPO-induced endomitosis in megakaryocytes.

Cite This Study

Rojnuckarin et al. (1999) studied this question.

synapsesocial.com/papers/6a70099d26770c2b8de04946https://doi.org/10.1182/blood.v94.4.1273
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