Why the study?
Does A-ELISA improve the detection of hepatitis A virus in estuarine water samples compared to standard ELISA and radioimmunoassay?
Does A-ELISA improve the detection of hepatitis A virus in estuarine water samples compared to standard ELISA and radioimmunoassay?
A-ELISA provides a highly sensitive, specific, rapid, and cost-effective method without radiolabels for detecting hepatitis A virus in environmental water samples.
May enhance environmental HAV surveillance; leaves open routine adoption pending independent validation.
An amplified enzyme-linked immunosorbent assay (A-ELISA) for detecting and quantifying hepatitis A virus in estuarine water samples is described. The test was five times more sensitive than a standard ELISA and at least two times more sensitive than radioimmunoassay. Test sensitivity was unaffected by the procedures used to concentrate the virus in estuarine samples or by the presence of humic and tannic acids in test samples. Nonspecific reactions were not encountered with a number of enteroviruses or with a rotavirus. A high sensitivity and specificity combined with speed, low cost, and freedom from radiolabels made the A-ELISA useful for detecting hepatitis A virus in environmental samples. The virus was detected in 3 of 20 estuarine water samples examined by A-ELISA.
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Nasser et al. (1987) studied this question.
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