Immunofluorescent techniques can successfully detect and localize viral antigens in human fetal intestinal organ cultures, offering a method to investigate viral agents in gastrointestinal disease.
This ex vivo model enables targeted viral localization studies in intestinal tissue; leaves open translation to clinical GI disease investigation.
Viral antigens from viruses belonging to four different viral groups were detected directly in human fetal intestinal organ cultures by the application of immunofluorescent techniques. The time of appearance and the cellular localization of fluorescent-stainable antigen varied with the type of virus under investigation. After infection with adenovirus or with adeno-associated virus, fluorescent-stainable antigen was seen in the epithelial cells of the explants, though no light microscopic changes could be observed. In infection with herpes simplex virus and echovirus, fluorescence was noted in both the epithelium and the lamina propria, along with histological changes throughout the organ culture. These techniques offer promise for the investigation of possible viral agents implicated in gastrointestinal disease.
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Dolin et al. (1972) studied this question.
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