Key result
Indirect fluorescent-antibody test (IFAT) in microcultures achieved isolation frequencies comparable to tube cultures and identified viruses within 3 to 4 hours of cytopathogenic effect recognition.
IFAT allows for rapid identification of adenovirus and coxsackievirus in microcultures within 3-4 hours of cytopathogenic effect recognition.
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Hypothesis-generating for rapid viral ID in microcultures; prospective validation required before diagnostic adoption.
The indirect fluorescent-antibody test (IFAT), employing treated and standardized antiserum in a single pool, was earlier reported to have been used successfully for the preliminary identification of nine respiratory viruses in second to ninth passage multiplying in cells propagated on microscope slides. This report describes parameters affecting the isolation in first passage and the identification by IFAT of adenovirus types 4 and 7 and coxsackievirus type A21 present in stored clinical specimens inoculated into microcultures of WI-38 cells. Isolation frequency was comparable to that obtained in tube cultures, and identification by IFAT of viruses in microcultures could be accomplished in 3 to 4 hr after recognition of a cytopathogenic effect.
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Stevens et al. (1971) studied Respiratory viruses (adenovirus types 4 and 7, coxsackievirus type A21). Indirect fluorescent-antibody test (IFAT) in microcultures vs. Tube cultures was evaluated on Isolation frequency and identification time. Indirect fluorescent-antibody test (IFAT) in microcultures achieved isolation frequencies comparable to tube cultures and identified viruses within 3 to 4 hours of cytopathogenic effect recognition.
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