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May 1, 1973Canadian Journal of Microbiology

Specific identification of enteroviruses by immuno-electron microscopy using a serum-in-agar diffusion method

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Population

17 virus samples consisting of 11 different enterovirus types (crude cell-culture isolates)

Comparison

Immuno-electron microscopy using a serum-in-agar… vs Electron microscopy in the absence of antiserum

Design

Preclinical

Authors

NAN. AndersonIowa Academy of ScienceFDFrances W. DoaneUniversity of Toronto

Discussion

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Implication

May improve enterovirus detection specificity in research; leaves open clinical translation pending human studies.

Structured PICO

P
Population
17 virus samples consisting of 11 different enterovirus types (crude cell-culture isolates)
I
Intervention
Immuno-electron microscopy using a serum-in-agar diffusion method with typing sera incorporated in the agar
C
Comparator
Electron microscopy in the absence of antiserum
O
Outcome
Specific virus identification and detection sensitivitysurrogate

The serum-in-agar diffusion method for immuno-electron microscopy provides highly specific and sensitive identification of enteroviruses.

Cite This Study

Anderson et al. (1973) studied this question.

synapsesocial.com/papers/6a7e60633eabdb005bb29faahttps://doi.org/10.1139/m73-096
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Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Rapid detection of Australia/SH antigen and antibody by a simple and sensitive technique of immunoelectronmicroscopy1971 · 63 citations
  2. 2Agar Diffusion Method for Negative Staining of Microbial Suspensions in Salt Solutions1972 · 39 citations
  3. 3Immune Electron Microscopy of Rhinoviruses1972 · 29 citations
  4. 4A Theory of the Structure and Process of Formation of Antibodies<sup>*</sup>1940 · 841 citations