Why the study?
Does trypsin digestion followed by immunofluorescence allow for the detection of viral antigens in formalin-fixed human tissues?
Does trypsin digestion followed by immunofluorescence allow for the detection of viral antigens in formalin-fixed human tissues?
Trypsin digestion enables retrospective diagnosis of viral infections using immunofluorescence on standard formalin-fixed, paraffin-embedded tissue specimens.
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May facilitate retrospective viral diagnosis in archived tissues; leaves open standardization of protocols across virus types.
Barth et al. (1988) studied this question.
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