Key result
Trypsin treatment combined with ethanol-acetic acid or Bouin's fixation yields the brightest specific immunofluorescent staining.
Glycol methacrylate embedding with proper fixation and trypsin pretreatment allows for effective immunofluorescent identification of virus-infected cells and detailed histological study.
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May enhance immunofluorescence in research tissues; hypothesis-generating for clinical histopathology pending human validation.
Greenlee et al. (1982) studied Murine K-papovavirus infection. Trypsin pretreatment and specific fixatives (96% ethanol/1% glacial acetic acid or Bouin's solution) vs. Protease V (pronase) and other fixatives was evaluated on Specific immunofluorescent staining and histological detail preservation. Treatment of Bouin's fixed sections with 0.4% trypsin for 30 minutes and fixation in 96% ethanol/1% glacial acetic acid or Bouin's solution provided the brightest specific immunofluorescent staining.
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