Key result
Trypsin digestion enhances viral antigen detection in formalin-fixed tissues to match fresh frozen samples.
Why the study?
Fluorescent antibody staining of viral antigens in formalin-fixed, paraffin-embedded tissues is limited and requires enhancement methods.
Trypsin digestion enables effective fluorescent antibody staining of viral antigens in routinely processed formalin-fixed, paraffin-embedded tissues, including those stored for up to 15 years.
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May aid retrospective viral studies with archived tissues; leaves open validation for clinical use in humans.
Swoveland et al. (1979) studied Viral infections (measles, herpes simplex). Trypsin digestion vs. Acetone-fixed, freshly frozen tissues was evaluated on Detection of viral antigens by fluorescent antibodies. Trypsin digestion markedly enhanced the detection of viral antigens by fluorescent antibodies in formalin-fixed, paraffin-embedded tissues, yielding results comparable to fresh frozen tissues.
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