What question did this study set out to answer?

To construct and characterize viable chimeric viruses combining dengue type 4 virus with structural proteins of tick-borne encephalitis virus to explore live attenuated vaccine feasibility.

November 1, 1992Proceedings of the National Academy of SciencesOpen Access

Construction and characterization of chimeric tick-borne encephalitis/dengue type 4 viruses.

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Authors

APAlexander G. PletnevNational Institutes of Health MBM BrayNational Institutes of Health JHJohn W. HugginsUnited States Army Medical Research Institute of Infectious Diseases

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Implication

Preclinical study demonstrates protective immunity from a chimeric flavivirus in mice, highlighting a viable strategy for live attenuated vaccines.

Key Points

To construct and characterize viable chimeric viruses combining dengue type 4 virus with structural proteins of tick-borne encephalitis virus to explore live attenuated vaccine feasibility.
Substituted tick-borne encephalitis virus genes (capsid, pre-membrane, envelope, or NS1) into dengue type 4 cDNA vectors and transfected transcribed RNA into permissive simian cells.
Evaluated viral replication kinetics and plaque formation in both simian and mosquito cell cultures.
Inoculated suckling and adult mice intracerebrally and peripherally to determine neurovirulence, peripheral invasiveness, and protection against lethal tick-borne encephalitis challenge.
Recovered viable vTBE(ME)/DEN4 chimera that replicated efficiently with larger plaques in simian cells but exhibited restricted entry and replication in mosquito cells relative to parental dengue virus.
Intracerebral inoculation of vTBE(ME)/DEN4 caused fatal encephalitis in suckling and adult mice, whereas peripheral inoculation caused no disease.
Peripheral immunization with vTBE(ME)/DEN4 conferred 100% protection against subsequent challenge with 10^3 median lethal doses of tick-borne encephalitis virus, while dengue virus inoculation provided 0% protection.

Cite This Study

Pletnev et al. (1992) studied this question.

synapsesocial.com/papers/6a701349febe604dd708df8e https://doi.org/10.1073/pnas.89.21.10532

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Implication

Preclinical study demonstrates protective immunity from a chimeric flavivirus in mice, highlighting a viable strategy for live attenuated vaccines.

Construction and characterization of chimeric tick-borne encephalitis/dengue type 4 viruses.

Discussion

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Implication

Key Points

Cite This Study

Construction and characterization of chimeric tick-borne encephalitis/dengue type 4 viruses.

Discussion

Member takes

Implication

Key Points

Cite This Study

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