What question did this study set out to answer?

This research aims to define the mechanisms underlying Ca2+ homeostasis in unstimulated platelets.

Ca2+ homeostasis in unstimulated platelets.

View Full Paper

Ask AI

Bookmark

Authors

Randomized trial examines calcium exchange in unstimulated platelets, suggesting mechanisms for Ca2+ balance.

This research aims to define the mechanisms underlying Ca2+ homeostasis in unstimulated platelets.
Utilized 45Ca2+ to study calcium exchange kinetics in intact unstimulated platelets.
Measured cytosolic free Ca2+ concentration with quin2.
Identified intracellular calcium pools using differential permeabilization with digitonin.
Maximum rate of Ca2+ exchange was 2 pmol/10(8) platelets/min at extracellular Ca2+ concentrations significantly lower than plasma levels.
Two intracellular Ca2+ pools were identified: a rapidly exchanging pool with t 1/2 of 17 min and a slowly exchanging pool with t 1/2 of 300 min, both responding differently to extracellular Ca2+ levels.
Metabolic inhibitors decreased slowly exchangeable Ca2+ pool size but increased cytosolic free Ca2+ concentration, indicating a role in Ca2+ homeostasis.

Lawrence F. Brass (1984) studied this question.

View Full Paper

Ask AI

Bookmark