What question did this study set out to answer?

To evaluate rapid metabolic alterations and phosphate turnover in platelet membrane phospholipids following stimulation with adenosine diphosphate (ADP).

November 1, 1972British Journal of Haematology

Changes in ³²P‐Labelling of Platelet Phospholipids in Response to ADP

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Authors

JLJ. V. LloydSouth Australia Pathology ENE. E. NishizawaMcMaster University Medical Centre JHJayanta HaldarJawaharlal Nehru Centre for Advanced Scientific Research

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Implication

In vitro experimental study demonstrates increased phosphate turnover in rabbit platelet phospholipids following ADP exposure, indicating rapid membrane lipid metabolic changes during aggregation.

Key Points

To evaluate rapid metabolic alterations and phosphate turnover in platelet membrane phospholipids following stimulation with adenosine diphosphate (ADP).
Washed rabbit platelets were labelled in vitro with 32PO4 and subsequently stimulated with ADP or control solution.
Lipid extraction was performed 30 seconds post-stimulation, followed by the isolation of phospholipid classes via thin layer chromatography.
Radiolabel was predominantly incorporated into phosphatidic acid (PA), phosphatidylinositol (MPI), diphosphatidylinositol (DPI), and triphosphatidylinositol (TPI) in both groups.
ADP stimulation for 30 seconds increased 32P-labelling of PA, DPI, and TPI without altering their total overall mass, indicating an accelerated turnover of phosphate moieties.

Cite This Study

Lloyd et al. (1972) studied this question.

synapsesocial.com/papers/6a70310b6ceb2bbd16dfd1bc https://doi.org/10.1111/j.1365-2141.1972.tb07092.x

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Implication

In vitro experimental study demonstrates increased phosphate turnover in rabbit platelet phospholipids following ADP exposure, indicating rapid membrane lipid metabolic changes during aggregation.

Changes in ³²P‐Labelling of Platelet Phospholipids in Response to ADP

Discussion

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Implication

Key Points

Cite This Study

Changes in ³²P‐Labelling of Platelet Phospholipids in Response to ADP

Discussion

Member takes

Implication

Key Points

Cite This Study

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